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2013
Cui H, Okamoto Y, Yoshioka K, Du W, Takuwa N, Zhang W, Asano M, Shibamoto T, Takuwa Y. Sphingosine-1-phosphate receptor 2 protects against anaphylactic shock through suppression of endothelial nitric oxide synthase in mice. J Allergy Clin Immunol. 2013 Nov;132(5):1205-1214.e9. doi: 10.1016/j.jaci.2013.07.026. Epub 2013 Sep 8. PMID: 24021572 [PubMed - in process]

Hara A, Sakai N, Furuichi K, Sakai Y, Takeya M, Bucala R, Mukaida N, Takuwa Y, Matsushima K, Kaneko S, Wada T.CCL2/CCR2 augments the production of transforming growth factor-beta1, type 1 collagen and CCL2 by human CD45-/collagen 1-positive cells under high glucose concentrations. Clin Exp Nephrol. 2013 Apr 6. [Epub ahead of print] PMID: 23564379 [PubMed - as supplied by publisher]

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2012
Biswas K, Yoshioka K, Asanuma K, Okamoto Y, Takuwa N, Sasaki T, Takuwa Y. Essential role of class II phosphatidylinositol-3-kinase-C2α in sphingosine 1-phosphate receptor-1-mediated signaling and migration in endothelial cells. J Biol Chem. 2013 Jan 25;288(4):2325-39. doi: 10.1074/jbc.M112.409656. Epub 2012 Nov 28. PMID: 23192342 [PubMed - indexed for MEDLINE]

Yoshioka K, Yoshida K, Cui H, Wakayama T, Takuwa N, Okamoto Y, Du W, Qi X, Asanuma K, Sugihara K, Aki S, Miyazawa H, Biswas K, Nagakura C, Ueno M, Iseki S, Schwartz RJ, Okamoto H, Sasaki T, Matsui O, Asano M, Adams RH, Takakura N, Takuwa Y. Endothelial PI3K-C2α, a class II PI3K, has an essential role in angiogenesis and vascular barrier function.
Nat Med. 2012 Oct;18(10):1560-9. doi: 10.1038/nm.2928. Epub 2012 Sep 16. PMID: 22983395 [PubMed - indexed for MEDLINE]

Bruce CR, Risis S, Babb JR, Yang C, Kowalski GM, Selathurai A, Lee-Young RS, Weir JM, Yoshioka K, Takuwa Y, Meikle PJ, Pitson SM, Febbraio MA. Overexpression of sphingosine kinase 1 prevents ceramide accumulation and ameliorates muscle insulin resistance in high-fat diet-fed mice. Diabetes. 2012 Dec;61(12):3148-55. doi: 10.2337/db12-0029. Epub 2012 Sep 6. PMID: 22961081 [PubMed - indexed for MEDLINE]

Takuwa Y, Ikeda H, Okamoto Y, Takuwa N, Yoshioka K. Sphingosine-1-phosphate as a mediator involved in development of fibrotic diseases. Biochim Biophys Acta. 2013 Jan;1831(1):185-92. doi: 10.1016/j.bbalip.2012.06.008. Epub 2012 Jun 23. Review. PMID: 22735357 [PubMed - indexed for MEDLINE]

Takuwa Y, Okamoto Y, Yoshioka K, Takuwa N. Sphingosine-1-phosphate signaling in physiology and diseases. Biofactors. 2012 Sep-Oct;38(5):329-37. doi: 10.1002/biof.1030. Epub 2012 Jun 7. Review. PMID: 22674845 [PubMed - indexed for MEDLINE]

Kageyama Y, Ikeda H, Watanabe N, Nagamine M, Kusumoto Y, Yashiro M, Satoh Y, Shimosawa T, Shinozaki K, Tomiya T, Inoue Y, Nishikawa T, Ohtomo N, Tanoue Y, Yokota H, Koyama T, Ishimaru K, Okamoto Y, Takuwa Y, Koike K, Yatomi Y. Antagonism of sphingosine 1-phosphate receptor 2 causes a selective reduction of portal vein pressure in bile duct-ligated rodents. Hepatology. 2012 Oct;56(4):1427-38. doi: 10.1002/hep.25780. PMID: 22505286 [PubMed - indexed for MEDLINE]

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2011
Takuwa N, Du W, Kaneko E, Okamoto Y, Yoshioka K, Takuwa Y. Tumor-suppressive sphingosine-1-phosphate receptor-2 counteracting tumor-promoting sphingosine-1-phosphate receptor-1 and sphingosine kinase 1 - Jekyll Hidden behind Hyde. Am J Cancer Res. 2011;1(4):460-81. Epub 2011 Feb 16. PMID: 21984966 [PubMed]

Okamoto Y, Yoshioka K, Takuwa N, Takuwa Y. [Cardiovascular function of sphingosine 1-phosphate signaling]. Seikagaku. 2011 Jun;83(6):536-44. Review. Japanese. No abstract available. PMID: 21800684 [PubMed - indexed for MEDLINE]

Ando K, Ishibashi T, Ohkawara H, Inoue N, Sugimoto K, Uekita H, Hu C, Okamoto Y, Takuwa Y, Takeishi Y. Crucial role of membrane type 1 matrix metalloproteinase (MT1- MMP) in RhoA/Rac1-dependent signaling pathways in thrombin- stimulated endothelial cells. J Atheroscler Thromb. 2011;18(9):762-73. Epub 2011 Jun 1. PMID: 21628952 [PubMed - indexed for MEDLINE]


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2010
Seok YM, Azam MA, Okamoto Y, Sato A, Yoshioka K, Maeda M, Kim IK, Takuw Y. Enhanced Ca2+-Dependent Activation of PI3KC2 alpha Rho Axis in Blood Vessels of Spontaneously Hypertensive Rats. Hypertension 56(5):934-941. 2010

H Suzuki, H Hashimoto, M Kawasaki, M Watanabe, H Otsubo, T Ishikura, H Fujihara, H Ohnishi, E Onuma, H Yamada-Okabe, Y Takuwa, E Ogata, T Nakamura, Y Ueta. Similar changes of hypothalamic feeding-regulating peptides mRNAs and plasma leptin levels in PTHrP-,LIF-secreting tumors-induced cachectic rats and adjuvant arthritic rats. Int J Cancer. 2010 In press.

H Ohkawara, T Ishibashi, S Saitoh, N Inoue, K Sugimoto, M Kamioka, H Uekita, T Kaneshiro, K Ando, Y Takuwa, Y Maruyama, Y Takeishi. Preventive effects of pravastatin on thrombintriggered vascular responses via Akt/eNOS and RhoA/Rac1 pathways in vivo. Cardiovasc. Res. 2010 In press.

Y Takuwa, W Du, X Qi, Y Okamoto, N Takuwa, K Yoshioka. Roles of sphingosine-1-phosphate signaling in angiogenesis. World J Biol Chem. 1(10):298-306, 2010

Y. Yoshida, M. Nakada, N. Sugimoto, T. Harada, Y. Hayashi, D. Kita, N. Uchiyama, Y. Hayashi, A. Yachie, Y. Takuwa, JI. Hamada. Sphingosine-1-phosphate receptor type 1 regulates glioma cell proliferation and correlates with survival of patients with glioblastoma. Int J Cancer. 126(10):2341-2352. 2010

Xun Qi, Yasuo Okamoto, Tomomi Murakawa, Fei Wang, Osamu Oyama, Ryunosuke Ohkawa, Kazuaki Yoshioka, Wa Du, Naotoshi Sugimoto, Yutaka Yatomi, Noriko Takuwa, Yoh Takuwa. Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice. Eur J Pharmacol. 2010 May 25;634(1-3):121-31.

Du W, Takuwa N, Yoshioka K, Okamoto Y, Gonda K, Sugihara K, Fukamizu A, Asano M, Takuwa Y. S1P2, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis and tumor growth in vivo in mice. Cancer Research 2010;70: 772 -781

N. Takuwa, S. Ohkura, S. Takashima, K. Ohtani, Y. Okamoto, T. Tanaka, K, Hirano, S. Usui, F. Wang, W. Du, K. Yoshioka, Y. Banno, M. Sasaki, I. Ichi, M. Okamura, N. Sugimoto, K. Mizugishi, Y. Nakanuma, I. Ishii, M. Takamura, S. Kaneko, S. Kojo, K. Satouchi, K.
Mitumori, J. Chun, Y. Takuwa S1P3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species. Cardiovasc Res 85(3):484-93. 2010



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2009
Y. Takuwa, K. shioka, Y. Okamoto , N. Takuwa The role of class II PI 3-kinase signaling
pathway in physiology and diseases. Curr Biodata Targeted Proteins Database. In press.
Bloggs, J. (2009) The Role of Class II PI 3-kinase Signaling in Physiology and Disease
Targeted Proteins Database 1, [22910], 10.2970/tpdb.2009.246.

A. Shiratsuchi, M. Ichiki, Y. Okamoto, N. Ueda, N. Sugimoto, Y. Takuwa, Y. Nakanishi
Inhibitory effect of N-palmitoylphosphatidylethanolamine on macrophage phagocytosis
through inhibition of Rac1 and Cdc42 J. Biochem. 145(1)43-50, 2009

K. Yonesu, Y. Kawase, T. Inoue , N. Takagi, J. Tsuchida, Y. Takuwa, S. Kumakura, F. Nara.
Involvement of sphingosine-1-phosphate and S1P(1) in angiogenesis: Analyses using a new
S1P(1) antagonist of non-sphingosine-1-phosphate analog. Biochem Pharmacol.
15;77(6):1011-1020. 2009

K. Sugimoto, T. Ishibashi, T. Sawamura, N. Inoue, M. Kamioka, H. Uekita, H. Ohkawara, T.
Sakamoto, N. Sakamoto, Y. Okamoto, Y. Takuwa, A. Kakino, Y. Fujita, T. Tanaka, T.
Teramoto, Y. Maruyama, Y. Takeishi LOX-1-LOX-1-MT1-MMP axis is crucial for RhoA and Rac1
activation induced by oxidized low-d nsity lipoprotein in endothelial cells Cardiovasc
Res. 84(1):127-36. 2009

H. Zhong, O. Matsui, K. Xu, T. Ogi, J. Sanada, Y. Okamoto, Y. Tabata, Y. Takuwa. Gene
transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel-coated
polyester stent-graft. J Vasc Surg 50(6):1433-43. 2009

Y. Yoshida, M. Nakada, N. Sugimoto, T. Harada, Y. Hayashi, D. Kita, N. Uchiyama, Y.
Hayashi, A. Yachie, Y. Takuwa, JI. Hamada Sphingosine-1-phosphate receptor type 1
regulates glioma cell proliferation and correlates with survival of patients with
glioblastoma. Int J Cancer 2009 in press

W Du, N Takuwa, K Yoshioka, Y Okamoto, K Gonda, K Sugihara, A Fukamizu, M Asano, Y Takuwa
S1P2, the G protein-coupled receptor for sphingosine-1-phosphate,negatively regulates
tumor angiogenesis and tumor growth in vivo in mice. 2009 Cancer Res In press.

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2008
O. Oyama, N. Sugimoto, X. Qi, N. Takuwa, K. Mizugishi, J. Koizumi, Y. Takuwa. The
lysophospholipid mediator sphingosine-1-phosphate promotes angiogenesis in vivo in
ischemic hindlimbs of mice. Cardiovasc Res. 78. 301-307, 2008

Y. Takuwa, Y. Okamoto, K. Yoshioka, N. Takuwa Sphingosine-1-phosphatesignaling and
biological activitiesin the cardiovascular system. Biochim. Biophys. Acta 1781(9):483-488,2008

S. Takashima, N. Sugimoto, N. Takuwa, Y. Okamoto, K. Yoshioka, M. Takamura, S. Takata, S.
Kanekob and Y. Takuwa. G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration
in vascular smooth muscle in a manner dependenton Rho but not Rho kinase. Cardiovasc Res
79(4):689-697,2008

M. Takabatake, T. Takuwa, N.Takuw, H.Yasuno, S. Matsumoto, M. Shibutani, K. Mitsumori. A
case report of a renal mixed epithelial and stromal tumor in a heterozygous S1P2 receptor
deficient mouse. J Vet Med Sci. 70(5): 483-485, 2008




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2007
T. Nakamura, K. Yonesu, Y. Mizuno, C. Suzuki, Y. Sakata, Y. Takuwa, F. Nara, S. Satoh, Synthesis and SAR studies of a novel class of S1P(1) receptor antagonists. Bioorg Med Chem. 2007 Mar 3; [In print][PubMed]


K. Yoshioka, N. Sugimoto, N. Takuwa, Y. Takuwa, Essential role for class II phosphoinositide 3-kinase alpha-isoform in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin phosphatase and contraction in isolated vascular smooth muscle cells. Mol Pharmacol. 2007;71(3):912-920.[PubMed]

H. Hashimoto, Y. Azuma, M. Kawasaki, H. Fujihara, E .Onuma, H. Yamada-Okabe, Y. Takuwa, E. Ogata, and Y. Ueta, Parathyroid Hormone-Related Protein Induces Cachectic Syndromes without Directly Modulating the Expression of Hypothalamic Feeding-Regulating Peptides. Clin Cancer Res 2007;13: 292-298.
[PubMed]

Sakamoto T, Ishibashi T, Sugimoto K, Sakamoto N, Ohkawara H, Niinuma M, Nagata K, Kamioka M, Sugimoto N, Watanabe A, Kurabayashi M, Takuwa Y, Maruyama Y. RhoA-dependent PAI-1 gene expression induced in endothelial cells by monocyte adhesion mediates geranylgeranyl transferase I and Ca2+ signaling. Atherosclerosis. 2007 ;193(1):44-54.

Yano M, Matsumura T, Senokuchi T, Ishii N, Murata Y, Taketa K, Motoshima H, Taguchi T, Sonoda K, Kukidome D, Takuwa Y, Kawada T, Brownlee M, Nishikawa T, Araki E. Statins activate peroxisome proliferator-activated receptor gamma through extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase-dependent cyclooxygenase-2 expression in macrophages. Circ Res. 2007; 100(10):1442-1451.

Ueta Y, Hashimoto H, Onuma E, Takuwa Y, Ogata E. Hypothalamic Neuropeptides and Appetite Response in Anorexia-Cachexia Animal. Endocr J. 2007;54(6):831-838

Oyama O, Sugimoto N, Qi X, Takuwa N, Mizugishi K, Koizumi J, Takuwa Y. The lysophospholipid mediator sphingosine-1-phosphate promotes angiogenesis in vivo in ischemic hindlimbs of mice. Cardiovasc Res. 2008 inpress.

Masayoshi Takabayake, Yoh Takuwa, Noriko Takuwa, Hironobu Yasuno, Sayaka Matsumoto, Makoto Sshibutani and Kunitoshi Mitsumori, A Case report of a renal mixed epithelial and stromal tumor in a heterozygous S1P2 receptor deficient mouse. J. Veterinary Medical Sciences 2008 In press

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2006
MA. Azam, K. Yoshioka, S. Ohkura, N. Takuwa, N. Sugimoto, K. Sato, Y. Takuwa, Ca2+-dependent, inhibitory effects of cyclic adenosine 5'-monophosphate on Ca2+ regulation of phosphoinositide 3-kinase C2a, Rho, and myosin phosphatase in vascular smooth muscle. Mol Pharmacol. 2007;71(3):912-920.[PubMed]

I. Inoki, N. Takuwa, N. Sugimoto, K. Yoshioka, S. Takata, S. Kaneko and Y. Takuwa, Negative regulation of endothelial morphogenesis and angiogenesis by S1P2 receptor. Biochem. Biophys. Res. Commun. 2006;346:293-300
[PubMed]

Y. Wang, K. Yoshioka, MA. Azam, N. Takuwa, S. Sakurada, Y. Kayaba, N. sugimoto, I. Inoki, T. Kimura, T. Kuwai and Y. Takuwa, Class II phosphoinositide 3-kinase alfa-isoform regulates Rho, myosin phosphatase and contraction in vascular smooth muscle. Biochem J 2006; 394: 581-592
[PubMed]

Y. Takuwa, N. Sugimoto, N.Takuwa, and Y. Igarasi, Signaling mechanisms for positive and negative regulation of cell motility by sphingosine-1-phosphate receptors. Sphingolipid Biology. 2006; pp. 415-425 Y. Hirabayashi, Y. Igarashi, A.H. Merrill(Eds.) Springer

Zheng R, Iwase A, Shen R, Goodman OB, Sugimoto N, Takuwa Y, Lerner DJ, Nanus DM., Neuropeptide-stimulated cell migration in prostate cancer cells is mediated by RhoA kinase signaling and inhibited by neutral endopeptidase.Oncogene. 2006; 25(44), 5942-5952 [PubMed]

Tanabe A, Kamisuki Y, Hidaka H, Suzuki M, Negishi M, Takuwa Y. 
PKC phosphorylates MARCKS Ser159 not only directly but also through RhoA/ROCK. Biochem Biophys Res Commun. 2006 Jun 23;345(1):156-61[
PubMed]

Naotoshi Sugimoto, Noriko Takuwa, Kazuaki Yoshioka and Yoh Takuwa Rho-dependent, Rho kinase-independent inhibitory regulation of Rac and cell migration by LPA
1 receptor in Gi-inactivated CHO sells. Exp. Cell Res. 2006; 312, 1899-1903[PubMed]
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2005
T Maruko, T Nakahara, K Sakamoto, M Saito,N Sugimoto, Y Takuwa and K Ishii Involvement of the subunits of G proteins in the cAMP response induced by stimulation of the histamine H
1 receptor. Naunyn-Schmiedegerg's Arch Pharmacol 2005;372:153-159[PubMed]

T. Sakamoto, T. Ishibashi, N. Sakamoto, K. Sugimoto, K. Egashira H. Ohkawara, K. Nagata, K. Yokoyama, M. Kamioka, T. Ichiki, N. Sugimoto, M. Kurabayashi, K. Suzuki, Y. Takuwa, and Y. Maruyama Endogenous NO Blockade Enhances Tissue Factor Expression via Increased Ca2+ Influx through MCP-1 in Endothelial Cells by Monocyte Adhesion.  Arterioscler Thromb Vasc Biol. 2005;25(9):2005-2011[
PubMed]

H. Ohtsu, M. Mifune, G. D. Frank, S. Saito, T. Inagami, S. K. Mitsuyama, Y. Takuwa, T. Sasaki, J. D. Rothstein, H. Suzuki, H. Nakashima, E. A. Woolfolk, E. D. Motley and S. Eguchi Signal-Crosstalk between Rho/ROCK and JNK mediates Migration of Vascular Smooth Muscle Cells stimulated by Angiotensin. Arterioscler Thromb Vasc Biol. 2005;25:1831-1836.[
PubMed]

H. Ohkawara, T. Ishibashi, T. Sakamoto, K. Sugimoto, K. Nagata, K. Yokoyama, N. Sakamoto, M. Kamioka, I. Matsuoka, S. Fukuhara, N. Sugimoto, Y. Takuwa and Y. Maruyama. Thrombin-induced rapid geranylgeranylation of RhoA as an essential process for RhoA activation in endothelial cells.  J Biol Chem. 2005;18;280(11):10182-10188.[
PubMed]

M. Philippova, D. Ivanov, R. Allenspach, Y. Takuwa, P. Erne and T. Resink. RhoA and Rac mediate endothelial cell polarication and detachment induced by T-cadherin. FASEB J. 19(6): 588-590, 2005. [
PubMed]

T. Senokuchi, T. Matsumura, M. Sakai, M. Yano, T. Taguchi, K. Sonoda, D. Kukidome, K. Imoto, T. Nishikawa, S. Kim-Mitsuyama, Y. Takuwa, E. Araki. Statins suppress oxidized low-density lipoprotein-induced macrophage proliferation by inactivation of small G protein p38 MAPK pathway. J. Biol. Chem. 2005, 280(8): 6627-6633.[
PubMed]

S. Nagasawa, N. Takuwa, N. Sugimoto, H. Mabuchi and Y. Takuwa. Inhibition of Rac activation as a mechanism for negativeregulation of actin cytoskeletal reorganization and cell motility by cyclic AMP. Biochem J. 385:737-744. 2005[
PubMed]
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2004
K. Sakurai, L. Cominacini, U. Garbin, A.F. Pasini, N. Sasaki, Y. Takuwa, T. Masaki, T. Sawamura. Induction of endothelin-1 production on endothelial cells via co-operative action between CD40 and lectin-like oxidized LDL receptor (LOX-1). J Cardiovasc. Pharmacol. 44: S173-S180, 2004.[
PubMed]

K. Sakurai, Y. Matsuo, T. Sudo, Y. Takuwa, S. Kimura, Y. Kasuya. Role of p38 mitogen-activated protein kinase in thrombus formation. J Recept Signal Transduct Res. 24(4): 283-296, 2004.[
PubMed]

D. Shida, J. Kitayama, H. Yamaguchi, K. Hama, J. Aoki, H. Arai, H. Yamashita, K. Mori, A. Sako, T. Konishi, T. Watanabe, T. Sakai, R. Suzuki, H. Ohta, Y. Takuwa and H. Nagawa. Dual mode regulation of migration by lysophosphatidic acid in human gastric cancer cells. Exp Cell Res. 301(2):168-78, 2004.[
PubMed]

M. Yamada, Y. Banno, Y. Takuwa, M. Koda, A. Hara and Y. Nozawa. Overexpression of phospholopase D prevents actinomycin D induced apoptosis through potentiation of phosphoinositide 3-kinase signaling pathways in Chinese hamster ovary cells. Biochem. J., 378(Pt2):649-656 2004. [
PubMed]

S. Usui, N. Sugimoto, N. Takuwa, S. Sakagami, S. Takata, S. Kaneko and Y. Takuwa. Boold lipid mediator sphingosine-1-phosphate potently stimulates platelet derived growth factor-A and -B chain expression through S1P1-Gi-Ras-MAPK-dependent induction of kruppel-like factor 5. J. Biol. Chem.
279, 12300-12311, 2004. [PubMed]
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2003
Y. Takuwa. Regulation of the Rho signaling pathway by excitatory agonists in vascular smooth muscle. Advances in Experimental Medicine and Biology.
538: 67-75, 2003. [PubMed]

S. Sakurada, N. Takuwa, N. Sugimoto, Y, Wang, M. Seto, Y. Sasaki and Y. Takuwa. Ca
2+-dependent activation of Rho and Rho kinase in membrane depolarization-and receptor stimulation-induced vascular smoth muscle contraction. Circ. Res. 93: 548-556, 2003. [PubMed]

H. Yamaguchi, J. Kitayama, N. Takuwa, K. Arikawa, I. Inoki, K. Takehara, H. Nagawa and Y. Takuwa. Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and hematogenous metastasis of melanoma cells. Biochem. J.
374: 715-722, 2003. [PubMed]

K. Arikawa, N. Takuwa, H. Yamaguchi, N. Sugimoto, J. Kitayama, H. Nagawa, K. Takehara and Y. Takuwa. Ligand-dependent inhibition of B16 melanoma cell migration and invasion via endogenous S1P2 G protein-coupled receptor requirement of inhibition of cellular Rac activity. J. Biol. Chem.
278: 32841-32851, 2003. [PubMed]

J. Xu, F. Wang, A. V. Keymeulen, P. Herzmark, A. Straight, K. Kelly, Y. Takuwa, N. Sugimoto, T. Mitchison, and H. R. Bourne. Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils. Cell
114: 201-214, 2003. [PubMed]

T. Ishibashi, T. Sakamoto, H. Ohkawara, K. Nagata, K. Sugimoto, S. Sakurada, N. Sugimoto, A. Watanabe, K. Yokoyama, N. Sakamoto, M. Kurabayashi, Y. Takuwa and Y. Maruyama. Integral role of RhoA activation in monocyte adhesion-tringgered tissue factor expression in endothelial cells. Arteriuscl. Thromb. Vascu. Biol.
23: 681-687, 2003. [PubMed]

D. Shida, J. Kitayama, H. Yamaguchi, Y. Okaji, NH. Tsuno, T. Watanabe, Y. Takuwa and H. Nagawa. Lysophosphatidic acid enhances metastatic potential of human colon carcinoma DLD1 cells through LPA1. Cancer Res.
63: 1706-1711, 2003. [PubMed]

N. Sugimoto, N. Takuwa, H. Okamoto, S. Sakurada and Y. Takuwa. Inhibitory and stimulatory regulation of Rac and cell motility by the G
12/13-Rho-and the Gi-pathways integrated downstream of a single G protein coupled sphingosine-1-phosphate receptor isoform. Mol. Cell. Biol. 23: 1534-1545, 2003. [PubMed]

K. Ito, E. Shimomura, T. Iwanaga, M. Shiraishi, K. Shido, J. Nakamura, H. Nagumo, M. Seto, Y. Sasaki and Y. Takuwa. Essentioal role of rho kinase in the Ca
2+ sensitization of prostaglandin F2a-induced contraction of rabbit aortae. J. Physiol. 546: 823-836, 2003. [PubMed]

H. Ikeda, H. Satoh, M. Yanase, Y. Inoue, T. Tomiya, M. Arai, K. Tejima, K. Nagashima, H. Maekawa, N. Yahagi, Y. Tamtomi, S. Sakurada, Y. Takuwa, I. Ogara, S. Kimura and K. Fujiwara. Antiproliferative action of sphingosine 1-phosphate in rat hepatocytes involves activation of Rho via Edg-5. Gastroenterology.
124: 459-469, 2003. [PubMed]

Y. Banno, Y. Takuwa, M. Yamada, N. Takuwa, K. Ohguchi, A. Hara and Y. Nozawa. Involvement of phospholipase D in insulin-like growth factor-I-induced activation of extracellular signal-regulated kinase, but not phosphatidylinositol 3-kinase or Akt, in chinese hamster ovary cells. Biochem. J.
369: 363-368, 2003. [PubMed]

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2002
K. Nagata, T. Ishibashi, T. Sakamoto, H. Ohkawara, J. Shido, K. Yokoyama, K. Sugimoto, S. Sakurada, S. Nakamura, Y. Maruyama. Rho/Rho-kinase is involved in the synthesis of tissue factor in human monocytes. Atherosclerosis.
163(1): 39-47, 2002. [PubMed]

T. Ishibashi, K. Nagata, H. Ohkawa, T. Sakamoto, K. Yokoyama, J. Shido, K. Sugimoto, S. Sakurada, T. Teramoto and Y. Maruyama. Inhibition of Rho/Rho-kinase signaling downregulates plasminogen activator inhibitor-1 synthesis in cultured human monocytes. Biophys. Biochim. Acta.
1590(1-3): 123-130, 2002. [PubMed]

Y. Takuwa, N. Takuwa, N. Sugimoto. The Edg G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities. J. Biochem.
131: 767-771, 2002. [PubMed]

K. Takami, N. Takuwa, H. Okazaki, M. Kobayashi, T. Ohtoshi, S. Kawasaki, M. Dohi, K. Yakamoto, T. Nakamura, M. Tanaka, K. Nakahara, Y. Takuwa and H. Takizawa. Interferon-g inbits hepatocyte growth factor-stimulated cell proliferation of human bronchial epithelial cells: upregulation of p27
kip1 cyclin-dependent kinase inhibitor. Am. J. Respir. Cell. Mol. Biol. 26(2): 231-238, 2002. [PubMed]

Y. Takuwa. Subtype-specific differential regulation of Rho family G proteins and cell migration by the Edg family sohingosine-1-phosphate receptors. Biophys. Biochim. Acta.
1582(1-3): 112-120, 2002. [PubMed]

Y. Ryu, N. Takuwa,
N. Sugimoto , S. Sakurada, S. Usui, H. Okamoto, O. Matsui and Y. Takuwa. Sphingosine-1-Phosphate, a Platelet-Derived Lysophospholipid Mediator, Negatively Regulates Cellular Rac Activity and Cell Migration in Vascular Smooth Muscle Cells. Circ Res. 90: 325-332, 2002. [PubMed]

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2001
Y. Banno, Y. Takuwa, Y. Akao, H. Okamoto, Y. Osawa, S. Nakashima, P. G. Suh, Y. Nozawa. Involvement of phospholipase D in sphingosine 1-phosphate-induced activation of phosphatidylinositol 3-kinase and Akt in chinese hamster ovary cells overexpressing EDG3. J. Biol. Chem.
276: 35622-35628, 2001. [PubMed]

S. Sakurada, H. Okamoto, N. Takuwa, N. Sugimoto and Y. Takuwa.Rho activation in evcitatory agonist-stimulated vascular smooth muscle. Am. J. Physiol.(Cell Physiol)
281: C571-C578, 2001. [PubMed]

H. Mitui, N. Takuwa, T. Maruyama, H. Maekawa, M. Hrayama, T. Sawatari, N. Hashimoto, Y. Takuwa and S Kimura. The MEK1-ERK map kinase pathway and the PI 3-kinase-AKT pathway independently mediate anti-apoptotic signals in HEPG2 liver cancer cells. Int. J. Cancer.
92: 55-62, 2001. [PubMed]

Y. Takuwa, H. Okamoto, N. Takuwa, K. Gonda, N. Sugimoto and S. Sakurada.Subtype-Specific, Differential Activities of the EDG Family Receptors for Sphingosine-1-Phosphate, a Novel Lysophospholipid Mediateor. Mol. Cell. Endocrinol.
177: 3-11, 2001. [PubMed]

O. Shido, S. Sakurada, N. Sugimoto, Y. Hiratsuka and Y. Takuwa. Ambient temperatures preferred by hummans acclimated to heat given at a fixed daily time. Physiology & Behavior
72: 387-392, 2001. [PubMed]

N. Takuwa and Y. Takuwa. Regulation of cell cycle molecules by the Ras effector system. Mol. Cell. Endocrinol.
177: 25-33, 2001. [PubMed]

shadow2
2000
H. Okamoto, N. Takuwa, T. Yokomizo, N. Sugimoto, S. Sakurada, H. Shigematsu and Y. Takuwa. Inhibitory Regulation of Rac Activation, Membrane Ruffling and Cell Migration by Sphingosine-1-Phosphate Receptor EDG5, but not EDG1 or EDG3. Mol.Cell.Biol.
20(24): 9247-9261, 2000. [PubMed]

H. Ikeda, Y. Yatomi, M. Yanase, H. Satoh, H. Maekawa, I. Ogara, Y. Ozaki, Y. Takuwa, S. Mochida and K. Fujiwara. Biological Activities of a Novel Lipid Mediator, Sphingosine 1-phosphate, in Rat Hepatic Stellate Cells. Am. J. Physiol.
279: G304-G310, 2000. [PubMed]

Kachigian, Y. Takuwa, T. Collins. Mechanisms of AngiotensinII-Induced Platelet-Derived Growth Factor Gene Expression. Mol. Cell. Biolchem.
212(1-2): 183-186, 2000. [PubMed]

H. Nagumo, Y. Sasaki, Y. Ono, H. Okamoto, M. Seto and Y. Takuwa. Rho kinase inhibitor HA1077 prevents Rho-mediated myosin phosphatase inhibition in smooth muscle cells. Am. J. Physiol.
278: C57-C65, 2000. [PubMed]

N. Sugimoto, S. Sakurada and O. Shido. Changes in ambient temperature at the onset of thermoregulatory responses in exercise-trained rats. Int. J. Biometeorol.
43(4): 169-71, 2000. [PubMed]

S. Sakurada, O. Shido, N. Sugimoto, Y. Hiratsuka, T. Yoda and K. Kanosue. Autonomic and behavioural thermoregulation in starved rats. J. Physiol.
526 Pt 2: 417-24, 2000. [PubMed]

Al. Ivanov , VA. Kulchitsky, N. Sugimoto, CT. Simons and AA. Romanovsky. Does the formation of lipopolysaccharide tolerance require intact vagal innervation of the liver? Auton Neurosci.
85(1-3): 111-8, 2000. [PubMed]


1999
N. Yukimasa, K Isobe, H Nagai, Y Takuwa, and T Nakai. Successive occupancy by immediate early transcriptional factors of the tyrosine hydroxylase gene TRE and CRE sites in PACAP-stimulatedPC12 pheochromocytoma cells. Neuropeptides.
33(6): 475-482, 1999. [PubMed]

J. Deguchi, J. Abe, M. Makuuchi and Y. Takuwa. Inhibitory effects of trapidil on PDGF signaling in balloon-injurede rat carotid artery. Life Sci.
65(26): 2791-2799, 1999. [PubMed]

J. Deguchi, M. Makuuchi, T. Nakaoka, T. Collins and Y. Takuwa. AngiotensinII stimulates platelet-derived growth factor-B chain expression in newborn rat vascular smooth muscle cells and neointimal cells through Ras, ERK, and JNK mechanisms. Cir. Res.
85: 565-574, 1999. [PubMed]

Y. Takuwa. Regulation of smooth muscle myosin phosphatase and contraction by Rho and Rho kinase. In Molecular mechanism and the disorder of smooth muscle edited by K. Kohama and Y. Sasaki. R. G. Landes company. Austin, Texas, U. S. A. 1999.

H. Okamoto, N. Takuwa, Y. Yatomi, K. Gonda, H. Shigematsu and Y. Takuwa. EDG3 is a functional receptor specific for sphingosine-1-phosphate and sphingosylphosphorylcholine with signaling characteristics distinct from EDG1 and AGR16. Biochem. Biophys. Res. Commun.
260(1): 203-208, 1999. [PubMed]

J. Deguchi, T. Nambu, H. Hamada, T. Nakaoka, J. Abe, O. Sato, T. Miyata, M. Makuuchi, K. Kurokawa and Y. Takuwa. Targeting endogenous platelet-growth factor B-chain by adenovirus-mediated gene transfer potently inhibits in vivo smooth muscle proliferation after arterial injury. Gene Therapy.
6(6): 956-965, 1999. [PubMed]

K. Gonda, H. Okamoto, N. Takuwa, Y. Yatomi, H. Okazaki, T. Sakurai, S. Kimura, R. Sillard, K. Harii and Y. Takuwa. The novel sphingosine 1-phosphate receptor AGR16 is coupled via pertussis toxin-sensitive and -insensitive G-proteins to multiple signalling pathways. Biochem. J.
337:67-75, 1999. [PubMed]

N. Takuwa, Y. Fukui and Y. Takuwa. Cyclin D1 Expression Mediated by Phosphatidylinositol 3-Kinase through mTOR-p70S6K-Independent Signaling in Growth Factor-Stimulated NIH 3T3 Fibroblasts. Mol.Cell.Biol.: 1346-1358, 1999. [
PubMed]

N. Sugimoto, C. T. Simons and A. A. Romanovsky. Vagotomy does not affect thermal responsiveness to intrabrain prostaglandin E2 and cholecystokinin octapeptide. Brain Res.
844: 157-163, 1999. [PubMed]

N. Sugimoto, S. Sakurada and O. Shido. Selected ambient temperatures of rats acclimated to heat given on various schedules. Pflugers Arch.
438(6):766-70, 1999. [PubMed]

N. Sugimoto, O. Shido, S. Sakurada and T. Nagasaka. Thermoregulatory responses to acute heat loads in rats following spontaneous running. Jpn. J. Physiol.
49: 47-53, 1999. [PubMed]

O. Shido, N. Sugimoto, M. Tanabe and S. Sakurada. Core temperature and sweating onset in humans acclimated to heat given at a fixed daily time. Am. J. Physiol.
276 (Regulatory Integrative Comp. Physiol. 45): R1095-R1101, 1999. [PubMed]

N. Sugimoto, O. Shido, F. Furuyama, S. Sakurada and H. Nishino. Thermoregulatory responses to acute heat load in the FOK rat. Int. J. Biometeorol.
43: 119-123, 1999. [PubMed]


1998
K. Hamada, N. Takuwa, K. Yokoyama and Y. Takuwa. Stretch activates jun N-terminal kinase/stress-activated protein kinase in vascular smooth muscle cells through mechanisms involving autocrine ATP stimulation of purinoceptors. J. Biol. Chem. 273(11); 6334-6340, 1998. [PubMed]

J. Abe, J. Deguchi, K. Hara, Y. Ikari, T. Tamura, M. Ohno, K. Kurokawa and Y. Takuwa. Tyrosine phosphorylation of platelet-derived growth factor b- receptors in coronary artery lesions: implications for vascular remodeling after directional coronary atherectomy and unstable angina pectoris. Heart. 79(4); 400-406, 1998. [PubMed]

H. Mitsui, T. Maruyama, S. Kimura and Y. Takuwa. Thrombin activates two stress-activated protein kinases, c-Jun N-terminal kinase and p38, in HepG2 cells. Hepatology. 27(5); 1362-1367, 1998. [PubMed]

Y. Takuwa. Regulation of smooth muscle myosin phosphatase and contraction by Rho and Rho kinase. In Molecular mechanism and the disorder of smooth muscle edited by K. Kohama. Springer, Tokyo. 1998. [PubMed]

H. Okamoto, N. Takuwa, K. Gonda, H. Okazaki, K. Chang, Y. Yatomi, H. Shigematsu and Y. Takuwa. EDG1 is a functional sphingosine-1-phosphate receptor that is linked via a Gi/o to multiple signaling pathways including phospholipase C activation, Ca2+ mobilization, Ras-MAPK activation, and adenylate cyclase inhibition. J.Biol. Chem. 273; 27104-27110, 1998. [
PubMed]

N. Sugimoto, O. Shido, S. Sakurada, S. Ito and T. Nagasaka. Changes in core temperature and thermoeffector thresholds in exercise-trained rats. Jpn. J. Physiol. 1998 Apr; 48(2): 163-6 [
PubMed]

C. T. Simons, V. A. Kulchitsky, N. Sugimoto, L. D. Homer, M. Szekely and A. A. Romanovsky. Signaling the brain in systemic inflammation: which vagal branch is involved in fever genesis? Am. J. Physiol. 1998 Jul; 275(1 Pt 2): R63-8 [PubMed]

A. A. Romanovsky, V. A. Kulchitsky, C. T. Simons and N. Sugimoto. Methodology of fever research: why are polyphasic fevers often thought to be biphasic? Am. J. Physiol. 1998 Jul; 275(1 Pt 2): R332-8 [PubMed]

M. F. Van-Oosterhout, F. W. Prinzen, S. Sakurada, R. W. Glenny and J. R. Hale. Fluorescent microspheres are superior to radioactive microspheres in chronic blood flow measurements. Am. J. Physiol. 1998 Jul; 275(1 Pt 2): H110-5 [PubMed]

O. Shido. Can our thermoregulatory system anticipate temperature exposure? Med. Hypotheses. 1998 Mar; 50(3): 199-202 [PubMed]

O. Shido, S. Sakurada, N. Sugimoto, F. Furuyama and T. Nagasaka. Thermoeffector thresholds and preferred ambient temperatures of the FOK rat. Am. J. Physiol. 274(3 Pt 2): R604-609, 1998. [PubMed]

A. A. Romanovsky, C. T. Simons, V. A. Kulchitsky, N. Sugimoto and M. Szekely. Vagus nerve in fever. Recent developments. Ann. N. Y. Acad. Sci. 856:298-299, 1998. [PubMed]

Sakurada. S and J. R. Hales. A role for gastrointestinal endotoxins in enhancement of heat tolerance by physical fitness. J. Appl. Physiol. 1998 Jan; 84(1): 207-14 [PubMed]

H. Mitsui, N. Takuwa, K. Kurokawa, J. H. Exton and Y. Takuwa. Dependence of activated Ga12-induced G1 to S phase cell cycle progression on both Ras/MAPK and Ras/Rac1/JNK cascades in NIH3T3 fibroblasts. J. Bio. Chem. 272 (8) 4904-4910, 1997. [PubMed]


1997
J. Abe, J. Deguchi, T. Matsumoto, N. Takuwa, M. Noda, M. Ohno, M. Makuuchi, K. Kurokawa, and Y. Takuwa. Stimulated activation of platelet-derived growth factor receptor in vivo in balloon-injured arteries - a link between angiotensin II and intimal thickening. Circulation 96(6): 1906-1913, 1997. [PubMed]

T. Yokomizo, T. Izumi, K. Chang, Y. Takuwa and T. Shimizu. A G-protein coupled receptor for leukotriene B4 that mediates chemotaxis. Nature 387: 620-624, 1997. [PubMed]

M. Hirakata, S. Kaname, U. Chung, N. Joki, Y. Hori, M. Noda, Y. Takuwa, T. Okazaki, T. Fujita, T. Katoh, and K. Kurokawa. Tyrosine kinase dependent expression of TGF- induced by stretch in mesangial cells. Kid. International 51: 1028-1036, 1997. [PubMed]

N. Takuwa and Y. Takuwa. Ras activity late in G1 phase required for p27Kip1 downregulation, passage through the restriction point, and entry into S phase in growth factor-stimulated NIH3T3 fibroblasts. Mol. Cell. Biol. 17(9): 5348-5358, 1997. [PubMed]

M. Noda, T. Katoh, K. Kurokawa and Y. Takuwa. Increased expression of parathroid hormone-related peptide gene in blood vessels of spontaneously hypertensive rats. Hypertension 30 (5): 1284-1288, 1997. [PubMed]

Y. Takuwa, K. Hamada, J. Abe, K. Chang, J. Deguchi and N. Takuwa. Functional roles of locally produced vasoactive substances in vascular responses to mechanical stress and injury. CVD Grand Round Series Vol. 3 (Ed. T. Asano, T. Hayakawa, T. Kirino, T. Ohta, K. Sano, T. Sasaki, K. Takakura and A. Tamura, Tokyo) Neuron Publishing Co., Ltd. Tokyo Vol 4 : 93-105, 1997. [PubMed]

T. Nakaoka, K. Gonda, T. Ogita, Y. Otawara-Hamamoto, F. Okabe, Y. Kira, K. Harii, K. Miyazono, Y. Takuwa and T. Fujita. Inhibition of rat vascular smooth muscle proliferation in vitro and in vivo by bone morphogenetic protein-2. J. Clin. Invest. 100( 11); 2824-2832, 1997. [PubMed]

A. A. Romanovsky, V. A. Kulchitsky, C. T. Simons, N. Sugimoto and M. Szekely. Cold defense mechanisms in vagotomized rats. Am. J. Physiol. 273(2 Pt 2): R784-789, 1997. [PubMed]

A. A. Romanovsky, V. A. Kulchitsky, C. T. Simons, N. Sugimoto and M. Szekely. Febrile responsiveness of vagotomized rats is suppressed even in the absence of malnutrition. Am. J. Physiol. 273(2 Pt 2): R777-783, 1997. [PubMed]

N. Sugimoto, O. Shido, S. Sakurada and T. Nagasaka. Lipopolysaccharide-induced fever in rats prolonged by a needle prick. Jpn. J. Physiol. 47(2): 199-204, 1997. [PubMed]

O. Shido, N. Sugimoto, S. Sakurada, Y. Kaneko and T. Nagasaka. Body core temperature of rats subjected to daily exercise limited to a fixed time. Int. J. Biometeorol. 40(3): 135-140, 1997. [PubMed]

A. A. Romanovsky, O. Shido, S. Sakurada, N. Sugimoto and T. Nagasaka. Endotoxin shock-associated hypothermia. How and why does it occur? Ann. N. Y. Acad. Sci. 15; 813: 733-737, 1997. [PubMed]

O. Shido, S. Sakurada, N. Sugimoto, F. Furuyama, H. Nishino and T. Nagasaka. Preferred ambient temperature and threshold temperatures for thermoregulation in the FOK rat. Ann. N. Y. Acad. Sci. 15; 813: 652-653, 1997. [PubMed]

N. Sugimoto, O. Shido, F. Furuyama, H. Nishino and T. Nagasaka. Heat loss responses to acute heat loads in the FOK rat. Ann. N. Y. Acad. Sci. 15; 813: 644-645, 1997. [PubMed]

J. R. Hales and S. Sakurada. Role of endotoxins in the enhanced heat tolerance of fit subjects. Ann. N. Y. Acad. Sci. 15; 813: 545-547, 1997. [PubMed]

N. Sugimoto, O. Shido, S. Sakurada, A. Hirai, Y. Kaneko and T. Nagasaka. How does heat acclimation after the preferred ambient temperatures of rats? In: Thermal Physiology 1997. Ed. By B. Nielsen Johannsen and R. Nielsen. Denmark: The August Krogh Institute. 91-94, 1997 [PubMed]

O. Shido, N. Sugimoto, S. Sakurada and T. Nagasaka. Body core temperature and threshold for sweating of humans after exposure to heat given daily at a fixed time. In: Thermal Physiology 1997. Ed. By B. Nielsen Johannsen and R. Nielsen. Denmark: The August Krogh Institute. 95-98, 1997. [PubMed]

V. A. Kulchisky, A. A. Romanovsky, C.T. Simons, N. Sugimoto and M. Szekely. The brown fat in the thermoregulatory response of vagotomized rats to lipopolysaccharide. In: Temperature Control in Health and Disease. Ed. By V. N. Gournie. Minsk: Institute of Physiology, National Academy of Sciences. 141-145, 1997. [PubMed]


1996
K. Hamada, N. Takuwa, W. Zhou, M. Kumada and Y. Takuwa. Protein kinase C inhibits the CAK-CDK2 cyclin-dependent kinase cascade and G1/S Cell cycle progression in human diploid fibroblasts. Biochem.Biophys.Acta. 1310: 149-156, 1996. [PubMed]

K. Isobe, N. Yukimasa, T. Nakai and Yoh Takuwa. Pituitary adenylate cyclase-activating polypeptide induces gene expression of the catecholamine sysnthesizing enzymes, tyrosine hydrozylase and dopamine beta-hydroxylase, through 3', 5'-cyclic adenosine monophosphate- and protein kinase C-dependent mechanisms in cultured porcine adrenal medullary chromaffin cells. Neuropeptides. 30: 167-175, 1996. [PubMed]

M. Noda, T. Yoh, T. Katoh and K. Kurokawa. Mechanical force regulation of vascular parathyroid hormon-related peptide expression. Kidney International 49: S154-S155, 1996. [PubMed]

Y. Takuwa. Regulation of vascular smooth muscle contraction: Ca2+, protein kinase C and myosin light chain phosphatase. Japan. Heart J. 37: 793-813, 1996. [PubMed]

N. Takuwa and Y. Takuwa. Signal transduction of the cell cycle regulation. its temporo-spacial architecture. Japan. J. Physiol. 46(6) 431-449, 1996 [PubMed]

K. Isobe, N. Yukimasa, T. Nakai and Yoh Takuwa. Pituitary adenylate cyclase-activating polypeptide induces gene expression of the catecholamine sysnthesizing enzymes, tyrosine hydrozylase and dopamine beta-hydroxylase in cultured porcine adrenal medullary chromaffin cells. Ann. N.Y. Acad .Sci. 805: 464-469, 1996. [PubMed]

N. Sugimoto, O. Shido, S. Sakurada and T. Nagasaka. Day-night variations of behavioral and autonomic thermoregulatory responses to lipopolysaccharide in rats. Jpn. J. Physiol. 1996 Dec; 46(6): 451-6 [PubMed]

A. A. Romanovsky, O. Shido, S. Sakurada, N. Sugimoto and T. Nagasaka. Endotoxin shock: thermoregulatory mechanisms. Am. J. Physiol. 1996 Apr; 270(4 Pt 2): R693-703 [PubMed]


1995
N. Takuwa, W. Zhou, amd Y. Takuwa. Calcium, calmodulin and cell cycle progression. Cellular Signalling 7: 93-104, 1995. [PubMed]

N. Takuwa, W. Zhou and Y. Takuwa. Involvement of intact inositol-1, 4, 5-trisphosphate-sensitive Ca2+ stores in cell cycle progression at the G1/S boundary in serum-stimulated human fibroblasts. FEBS Letters 360: 173-176, 1995. [PubMed]

M. Noda, C. Yasuda-Fukazawa, K. Morishi, T. Kato, T. Okuda, K. Kurokawa and Y. Takuwa. Involvement of rho in GTPgS-induced enhancement of phosphorylation of 20 kDa myosin light chain in vascular smooth muscle cells:Inhibition of phosphatase activity. FEBS Letters 367: 246-250, 1995. [PubMed]

G. Whitney, D. Throckmorton, C. Isales, Y. Takuwa, J. Yeh, H. Rasmussen and C. Brophy. Kinase activation and smooth muscle contraction in the presence and absence of calcium. J. Vascu. Surgery. 22: 37-44, 1995. [PubMed]

M. Noda, Y. Takuwa, T. Katoh and K. Kurokawa. Stretch-induced parathyroid hormone-related peptide gene expression: implication in the regulation of myogenic tone. Curr. Opin. Nephrol. Hypertension 4: 383-387, 1995. [PubMed]

K. Chang, K. Hanaoka, M. Kumada and Y. Takuwa. Molecular cloning and functional analysis of a novel P2 nucleotide receptor. J. Bio. Chem. 270: 26152-26158, 1995. [PubMed]

Y. Takuwa, M. Noda, C. Yasuda, M. Kumada and K. Kurokawa. Regulation of Ca2+-dependent phosphorylation of 20-kDa myosin light chain by the small molecular weight G protein rho p21 in vascular smooth muscle cells. In Nakano,T. and Hartshorne, D. J. (eds.), Regulation of the contractile cycle in smooth muscle. Springer. 103-110, 1995. [PubMed]

T. Kiyohara, S. Miyata, T. Nakamura, O. Shido, T. Nakashima and M. Shibata. Differences in Fos expression in the rat brains between cold and warm ambient exposures. Brain. Res. Bull. 1995; 38(2): 193-201. [PubMed]

S. Miyata, M. Ishiyama, O. Shido, T. Nakashima, M. Shibata and T. Kiyohara. Central mechanism of neural activation with cold acclimation of rats using Fos immunohistochemistry. Neurosci. Res. 1995 May; 22(2): 209-18. [PubMed]

O. Shido, S. Sakurada, N. Sugimoto and T. Nagasaka. Shifts of thermoeffector thresholds in heat-acclimated rats. J. Physiol. Lond. 1995 Mar 1; 483 ( Pt 2): 491-7. [PubMed]

N. Sugimoto, O. Shido and S. Sakurada. Thermoregulatory responses of rats acclimated to heat given daily at a fixed time. J. Appl. Physiol. 1995 May; 78(5): 1720-4. [PubMed]

S. Sakurada, O. Shido, K. Yamamoto, N. Sugimoto, T. Kobayashi and T. Nagasaka. Effect of digital nerve blockade on heat-induced vasoconstriction in the human finger. J. Appl. Physiol. 1995 Feb; 78(2): 746-9. [PubMed]


1994
J. Abe, W. Zhou, J. Taguchi, N. Takuwa, K. Miki, H. Okazaki, K. Kurokawa, M. Kumada and Y. Takuwa. Suppression of neointimal smooth muscle cell accumulation in vivo by antisense cdc2 and cdk2 oligonucleotides in rat carotid artery. Biochem. Biophys. Res. Commun. 198: 16-24, 1994. [PubMed]

W. Zhou, N. Takuwa, M. Kumada and Y. Takuwa. E2F1, B-myb and selective members of cyclin/CDK subunits are targets for protein kinase C-mediated bimodal growth regulation in vascular endothelial cells. Biochem. Biophys. Res. Commun. 199: 191-198, 1994. [PubMed]

N. Ishizaka, H. Okazaki, K. Kurokawa, M. Kumada and Y. Takuwa. Molecular cloning of a novel putative G protein-coupled receptor from rat aortic smooth muscle. Downregulation of the mRNA level by the cyclic AMP messenger pathway. Biochim. Biophys. Acta 1218: 173-180, 1994. [PubMed]

J. Abe, W. Zhou, N. Takuwa, J. Taguchi, K. Kurokawa, M. Kumada and Y. Takuwa. A fumagillin derivative angiogenesis inhibitor, AGM-1470, inhibits activation of cyclin-dependent kinases and phosphorylation of retinoblastoma gene product but not protein tyrosyl phosphorylation or protooncogene expression in vascular endothelial cells. Cancer Research 54: 3407-3712, 1994. [PubMed]

M. Noda, T. Katoh, N. Takuwa, M. Kumada, K. Kurokawa and Y. Takuwa. Synergistic stimulation of parathyroid hormone-related peptide gene expression by mechanical stretch and angiotensin II in rat aortic smooth muscle cells. J. Biol. Chem. 269: 17911-17917, 1994. [PubMed]


1993
N. Takuwa, W. Zhou, M. Kumada and Y. Takuwa. Ca2+- dependent stimulation of retinoblastoma gene product phosphorylation and p34cdc2 kinase activation in serum-stimulated human fibroblasts. J. Biol. Chem. 268: 138-145, 1993. [PubMed]

T. Asano, T. Mastui, T. Watanabe, T. Koide and Y. Takuwa. The pathogenetic mechanism underlying cerebral vasospasm. Cellular membrane a key to disease processes. (T. Ohnishi ed. CRC Press, Inc.) PP. 209-240, 1993. [PubMed]

H. Okazaki, N. Ishizaka, T. Sakurai, K. Kurokawa, K. Goto, M. Kumada and Y. Takuwa. Molecular cloning of a novel putative G protein-coupled receptor expressed in the cardiovascular system. Biochem. Biophys. Res. Commun. 190: 1104-1109, 1993. [PubMed]

K. Isobe, T. Nakai and Y. Takuwa. Ca2+- dependent stimulatory effect of pituitary adenylate cyclase-activating polypeptide on catecholamine secretion from cultured pocine adrenal medullary chromaffin cells. Endocrinology 132: 1757-1765, 1993. [PubMed]

Y. Takuwa, T. Matsui, Y. Abe, T. Nagafuji, K. Yamashita and T. Asano. Alterations in protein kinase C activity and membrane lipid metabolism in cerebral vasospasm after subarachnoid hemorrhage. J. Cereb. Blood Flow Metab. 13: 409-415, 1993. [PubMed]

Y. Takuwa. Endothelin in vascular and endocrine systems: biological activities and its mechanisms of action. Endocrine J. 40: 489-506, 1993. [PubMed]

W. Zhou, N. Takuwa, M. Kumada, Y. Takuwa. Protein kinase C-mediated bidirectional regulation of DNA synthesis, RB protein phosphorylation and cyclin-dependent kinases in human vascular endothelial cells. J. Biol. Chem. 268: 23041-23048, 1993. [PubMed]

J. Taguchi, J. Abe, H. Okazaki, Y. Takuwa and K. Kurokawa. L-arginine inhibits neointimal formation following balloon injury. Life Sciences 53: PL387-392, 1993. [PubMed]

J. Taguchi, J. Abe, H. Okazaki, M. Ochiai, M. Ohno, Y. Takuwa and K. Kurokawa. Angiotensin converting enzyme inhibitors or dup753 prevent neointimal formation following balloon injury with single topical or multiple systemic applocation. Biochem. Biophys. Res. Commun. 196: 969-974, 1993. [PubMed]


1992
N. Takuwa, W. Zhou, M. Kumada and Y. Takuwa. Ca2+/calmodulin is involved in growth factor-induced retinoblastoma gene product phosphorylation in human vascular endothelial cells. FEBS Letters 306: 173-175, 1992. [PubMed]

T. Sakurai, H. Morimoto, Y. Kasuya, Y. Takuwa, H. Nakauchi, T. Masaki and K. Goto. Level of ETB receptor mRNA is down-regulated by endothelins through decreasing the intracellular stability of mRNA molecules. Biochem. Biophys. Res. Commun. 186: 342-347, 1992. [PubMed]

Y. Kasuya, Y. Takuwa, M. Yanagisawa, T. Masaki and K. Goto. A pertussis toxin-sensitive mechanism of endothelin action in porcine coronary artery smooth muscle. Br. J. Pharmacol. 107: 456-462, 1992. [PubMed]

N. Takuwa, W. Zhou, M. Kumada and Y. Takuwa. Activators of protein kinase C induce p34cdc2 histone H1 kinase stimulation in Swiss 3T3 fibroblasts. Biochem. Biophys. Res. Commun. 188: 1084-1089, 1992. [PubMed]

K. Onozaki, K. Kato, S. Inui, Y. Takuwa and Y. Akiyama. Cyclic AMP mimics IL-1 action on augmenting the differentiation of a mouse myeloid leukemic cell line (M1). J. Parmacobio-Dyn. 15: 491-500, 1992. [PubMed]

T. Yamaoka, T. Kudo, Y. Takuwa, Y. Kawakami, M. Itakura and K. Yamashita. Hereditary adrenocoritical unresponsiveness to adrenocoritcopin with a postreceptor defect. J. Clin. Endocrin. Meta. 75: 270-274, 1992. [PubMed]


1991
Y. Takuwa, C. Ohse, E. A. Wang, J. M. Wozney and K. Yamashita. Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3-E1. Biochem. Biophys. Res. Commun. 174: 96-101, 1991. [PubMed]

N. Takuwa, A. Iwamoto, M. Kumada, K. Yamashita and Y. Takuwa. Role of Ca2+ influx in bombesin-induced mitogenesis in Swiss 3T3 fibroblasts. J. Biol. Chem. 266: 1403-1409, 1991. [PubMed]

T. Masaki, M. Yanagisawa, K. Goto, S. Kimura and Y. Takuwa. Cardiovascular significance of endothelin. Cardiovascular significance of endothelium-derived vasoactive factors. (G. M. Rubanyi ed. Future Publishing Co.) PP. 65-81, 1991. [PubMed]

T. Matsui, Y. Takuwa, H. Joshita, K. Yamashita and T. Asano. Possible role of protein kinase C-dependent smooth muscle contraction in the pathogenesis of chronic cerebral vasospasm. J. Cereb. Blood Flow Metab. 11: 143-149, 1991. [PubMed]

Y. Takuwa, T. Masaki and K. Yamashita. The mechanisms of endothelin action in vascular smooth muscle cells. Contributions Nephrol. 90: 99-104, 1991. [PubMed]

A. Sakamoto, M. Yanagisawa, T. Sakurai, Y. Takuwa, M. Yanagisawa and T. Masaki. Cloning and functional expression of human cDNA for the ETB endothelin receptor. Biochem. Biophys. Res. Commun. 178: 656-663, 1991. [PubMed]

N. Takuwa, M. Kumada, K. Yamashita and Y. Takuwa. Mechanisms of bombesin-induced arachidonate mobilization in Swiss 3T3 fibroblasts. J. Biol. Chem. 266: 14237-14243, 1991. [PubMed]

T. Matsui, M. Sugawa, H. Joshita, Y. Takuwa and T. Asano. Activation of the protein kinase C-mediated contractile system in canine basilar artery undergoing chronic vasospasm. Stroke 22: 1183-1187, 1991. [PubMed]

Y. Abe, Y. Kasuya, M. Kudo, K. Yamashita, K. Goto, T. Masaki and Y. Takuwa. Endothelin-1-induced phosphorylation of 20kDa myosin light chain and caldesmon in porcine coronary artery smooth muscle. Japan J. Pharmacol. 57: 431-435, 1991. [PubMed]


1990
Y. Takuwa, M. Yanagisawa, N. Takuwa and T. Masaki. Endothelin, its diverse biological activities and mechanisms of action. Progress Growth Factor Res. 1: 195-206, 1990. [PubMed]

Y. Takuwa, Y. Kasuya, N. Takuwa, M. Kudo, M. Yanagisawa, K. Goto, T. Masaki and K. Yamashita. Endothelin receptor is coupled to phospholipase C via a guanine nucleotide binding regulatory protein which is distinct from pertussis toxin substrates. J. Clin. Invest. 85: 653-658, 1990. [PubMed]

T. Matsui, Y. Takuwa, K. Yamashita and T. Asano. The protein kinase C-dependent contractile system participates in the occurrence of chronic vasospasm. Pharmacology of Cerebral Ischemia (J. Krieglstein, H. Oberpichler eds. Wissenschaftliche Verlagsgesellschaft) PP. 145-149, 1990. [PubMed]

Y. Takuwa and H. Rasmussen. Role of Ca2+ and protein kinase C in the smooth muscle contraction. Proceedings of the IVth International Conference on Cerebral Vasospasm (K. Sano, K. Takakura et al. eds. University of Tokyo Press) PP. 59-64, 1990.

T. Sakurai, M. Yanagisawa, Y. Takuwa, H. Miyazaki, S. Kimura, K. Goto and T. Masaki. Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor. Nature 348: 732-735, 1990. [PubMed]

T. Masaki, M. Yanagisawa, S. Kimura, Y. Takuwa and K. Goto. Effect of endothelin on cardiovascular system. Jpn. Heart J. 31: 517-518, 1990. [PubMed]

H. Rasmussen, H. Haller, Y. Takuwa, G. Kelley and S. Park. Messenger Ca2+, protein kinase C and smooth muscle contraction. Frontiers in Smooth Muscle Research PP. 89-106, 1990. [PubMed]

S. Nakamura, N. Kuzuya, Y. Koide, M. Matsumura, Y. Watanabe, H. Tomizawa, Y. Murayama, Y. Okuda, Y. Takuwa, M. Itakura, K. Kawai, E. Ueno, E. Akaogi, K. Fukao and K. Yamashita. Successful Treatment of a metastatic hormone-producing adrenal cancer by a combination of mitotane, tegafur and surgical resection. Endocrinol. Jpn. 37: 753-762, 1990. [PubMed]

Y. Takuwa, T. Masaki and K. Yamashita. The effects of the endothelin family peptides on cultured osteoblastic cells from rat calvariae. Biochem. Biophys. Res. Commun. 170: 998-1005, 1990. [PubMed]

T. Masaki, M. Yanagisawa, Y. Takuwa, K. Kasuya, S. Kimura and K. Goto. Cellular mechanism of vasoconstriction induced by endothelin. The Biology and Medicine of Signal Transduction. (Y. Nishizuka ed. Raven Press) PP. 425-428, 1990. [PubMed]

N. Takuwa, Y. Takuwa, Y. Ohue, H. Mukai, K. Endoh, K. Yamashita, M. Kumada and E. Munekata. Stimulation of calcium mobilization but not proliferation by bombesin and tachykinin neuropeptides in human small cell lung cancer cells. Cancer Res. 50: 240-244, 1990. [PubMed]


1989
Y. Takeuchi, T. Matsumoto, Y. Takuwa, Y. Hoshino, T. Kurokawa, N. Shibuya and E. Ogata. High incidence of obesity and elevated serum immunoreactive insulin level in patients with paravertebral ligamentous ossification. J. Bone Mineral Metab. 7: 17-21, 1989. [PubMed]

M. Yanagisawa, A. Inoue, Y. Takuwa, Y. Mitsui, M. Kobayashi and T. Masaki. The human preproendothelin-1 gene: possible regulation by endothelial phosphoinositide turnover signaling. J. Cardiovascuel. Pharmacol. 13(Suppl. 5): S13-S17, 1989. [PubMed]

A. Inoue, M. Yanagisawa, Y. Takuwa, Y. Mitsui, M. Kobayashi and T. Masaki. The human preproendothelin-1 gene. Complete nucleotide sequence and regulation of expression. J. Biol. Chem. 264: 14954-14959, 1989. [PubMed]

K. Goto, Y. Kasuya, N. Matsui, Y. Takuwa, H. Kurihara, T. Ishikawa, S. Kimura, M. Yanagisawa and T. Masaki. Endothelin activates the dihydropyridine-sensitive,voltage-dependent Ca2+ channel in vascular smooth muscle. Proc. Natl. Acad. Sci. USA. 86: 3915-3918, 1989. [PubMed]

Y. Kasuya, Y. Takuwa, M. Yanagisawa, S. Kimura, K. Goto and T. Masaki. Endothelin-1 induces vasoconstriction through two functionally distinct pathways in porcine coronary artery: contribution of phosphoinositide turnover. Biochem. Biophys. Res. Commun. 161: 1049-1055, 1989. [PubMed]

N. Takuwa, Y. Takuwa, M. Yanagisawa, K. Yamashita and T. Masaki. A novel vasoactive peptide endothelin stimulates mitogenesis through inositol lipid turnover in Swiss 3T3 fibroblasts. J. Biol. Chem. 264: 7856-7861, 1989. [PubMed]

Y. Takuwa, Y. Ohue and K. Yamashita. Endothelin-1 activates phospholipase C and mobilizes Ca2+ from extra- and intracellular pools in osteoblastic cells. Am. J. Physiol. 257: E797-E803, 1989. [PubMed]

N. Takuwa, M. Ganz, Y. Takuwa, R. B. Sterzel and H. Rasmussen. Studies of the mitogenic effect of serotonin in rat renal mesangial cells. Am. J. Physiol. 257: F431-F439, 1989. [PubMed]


1988
Y. Takuwa, N. Takuwa and H. Rasmussen. The effects of isoproterenol on intracellular Ca2+ concentration in bovine tracheal smooth muscle. J. Biol. Chem. 263: 762-768, 1988. [PubMed]

N. Takuwa, Y. Takuwa and H. Rasmussen. Stimulation of mitogenesis and glucose transport by 1-monooleoylglycerol in Swiss 3T3 fibroblasts. J. Biol. Chem. 263: 9738-9745, 1988. [PubMed]

M. Yamamoto, Y. Takuwa, S. Masiko and E. Ogata. Effects of endogenous and exogenous parathyroid hormone on tubular reabsorption of calcium in pseudohypoparathyroidism. J. Clin. Endocrinol. Metab. 66(3) 618-625, 1988. [PubMed]

H. Rasmussen, C. Isales, Y. Takuwa, N. Takuwa, P. Barrett and W. Zawalich. The cellular basis for short-term memory in endocrine systems. Cellular mechanisms of conditioning and behavioral plasticity (D. Woody, D. L. Alkon and J. L. McGaugh ed. Plenum Publishing Corporation) PP. 3-10, 1988. [PubMed]

S. Paglin, Y. Takuwa, K. E. Kamm, J. T. Stull, H. Garvas and H. Rasmussen. Atrial natriuretic peptide inhibits the agonist-induced increase in extent of myosin light chain phosphorylation in aortic smooth muscle. J. Biol. Chem. 263: 13117-13120, 1988. [PubMed]

Y. Takuwa, G. Kelley, N. Takuwa and H. Rasmussen. Protein phosphorylation changes in bovine carotid artery smooth muscle during contraction and relaxation. Mol. Cell. Endocrinol. 60: 71-86, 1988. [PubMed]


1987
Y. Takeuchi, T. Matsumoto,Y. Takuwa and E. Ogata. Effect of salmon calcitonin on malignancy-associated hypercalcemia. J. Bone Mineral Metab. 4: 1-8, 1987. [PubMed]

H. Rasmussen, P. Barrett, Y. Takuwa and W. Apfeldorf. Calcium in the regulation of aldosterone secretion and vascular smooth muscle contraction. Hypertension. 10(Suppl I): I-23-I-26, 1987. [PubMed]

N. Takuwa, Y. Takuwa and H. Rasmussen. The effects of bombesin on phosphoinositide and calcium metabolism in Swiss 3T3 cells. J. Biol. Chem. 262(1): 182-188, 1987. [PubMed]

W. Zawalich, N. Takuwa, Y. Takuwa, V. Diaz and H. Rasmussen. Interactions of cholecystokinin and glucose in rat pancreatic islets. Diabetes 36(4): 426-433, 1987. [PubMed]

N. Takuwa, Y. Takuwa and H. Rasmussen. A tumour promotor, 12-0-tetradecanoylphorbol 13-acetate, increases cellular 1,2-diacylglycerol content through a mechanism other than phosphoinositide hydrolysis in Swiss 3T3 fibroblasts. Biochem. J. 243(3) 647-653, 1987. [PubMed]

H. Rasmussen, S. Park, Y. Takuwa, N. Takuwa, W. Apfeldorf and P. Barrett. A temporo-spatial model of Ca2+ messenger action. Calcium regulation and bone metabolism: basic and clinical aspects (D. V. Chon, T. J. Martin, P. J. Meunier ed. Elsevier Science Publishers B. V. ) 9: 159-166, 1987. [PubMed]

H. Rasmussen, P. Barrett, W. Apfeldorf, Y. Takuwa, N. Takuwa, J. Smallwood,C. Isales, W. Bollag and P. Stein. Multiplicity of Ca2+ signal transduction pathways. Calcium-dependent processes in the liver. (C. Heilmann ed. MTP Press Limited) PP. 9-29, 1987. [PubMed]

Y. Takuwa and H. Rasmussen. Measurement of cytoplasmic free Ca2+ in rabbit aorta using the photoprotein, aequorin. Effect of atrial natriuretic peptide on agonist-induced Ca2+ signal generation. J. Clin. Invest. 80: 248-257, 1987. [PubMed]

Y. Takuwa, N. Takuwa and H. Rasmussen. Measurement of cytoplasmic free Ca2+ concentration in bovine tracheal smooth muscle using the photoprotein, aequorin. Am. J. Physiol. 253: C817-C827, 1987. [PubMed]

H. Rasmussen, Y. Takuwa and S. Park. Protein kinase C in the regulation of the smooth muscle contraction. FASEB J. 1: 177-185, 1987. [PubMed]


1986
Y. Takuwa, M. Yamamoto, T. Matsumoto, K. Hata and E. Ogata. Hyperphosphatemia after surgical correction of hypercortisolism in patients with Cushing's syndrome. Mineral Electrol. Metab. 12(2): 119-124, 1986. [PubMed]

Y. Takuwa and E. Ogata. Regulation of sodium-coupled phosphate transport by extracellular phosphate in cultured kidney cells (JTC-12). Clin. Sci. 71(2): 307-312, 1986. [PubMed]

Y. Takuwa, N. Takuwa and H. Rasmussen. Carbachol induces a rapid and sustained hydrolysis of polyphosphoinositide in bovine tracheal smooth muscle. Measurement of the mass of polyphosphoinositide, 1,2-diacylglycerol and phosphatidic acid. J. Biol. Chem. 261(3): 14670-14675, 1986.

H. Rasmussen, W. Apfeldorf, P. Barret, N. Takuwa, W Zawalich, D. Kreutter, S. Park, and Y. Takuwa. Inositol lipids: integration of cellular signalling systems. In: Phosphoinositides and Receptor Mechanisms (Alan R. Liss, Inc.) PP. 109-147, 1986.


1985
Y. Takuwa and E. Ogata. Characterization of Na+-dependent phosphate uptake in cultured kidney cells (JTC-12) from monkey. Biochem. J. 230(3): 715-721, 1985.

Y. Takuwa and E. Ogata. Differentiated properties characteristic of renal proximal epithelium in a cell line derived from a normal monkey kidney (JTC-12). In Vitro Cell Dev. Biol. 21(8): 445-449, 1985.

Y. Takuwa T. Matsumoto, T. Kurokawa, M. Iizuka, Y. Hoshino, K. Hata and E. Ogata. Calcium metabolism in paravertebral ligamentous ossification. Acta Endocrinol. 109(2): 428-432, 1985.

N. Yamashita, Y. Takuwa and E. Ogata. Growth hormone-releasing factor reduces voltage-gated Ca2+ channel current in rat GH3 cells. J. Membr. Biol. 87(3): 241-247, 1985.


1984
T. Matsumoto, Y. Takuwa, T. Okazaki, Y. Hoshino, M. Iizuka, T. Kurokawa, K. Hata and E. Ogata. Association of paravertebral ligamentous ossification with hypoparathyroidism. Endocrine control of bone and calcium metabolism. (In D. V. Cohn, T. Fujita, J. T. Potts Jr, R. V. Talmage) Elsevier Scienc B.V., 26-31, 1984.

T. Okazaki, Y. Takuwa, M. Yamamoto, T. Igarashi,T. Kurokawa and E. Ogata. Ossification of the paravertebral ligaments: A frequent complication of hypoparathyroidism. Metabolism. 33(8): 710-713, 1984.